The amplification of ribosomal RNA genes involves a rolling circle intermediate.
نویسندگان
چکیده
During the development of Xenopus oocytes there is a special DNA synthesis that leads to a thousandfold amplification of the genes that code for ribosomal RNA. We have used the electron microscope to study this process. Our primary observation is the presence of ribosomal DNA in rolling-circle intermediates at the time of amplification. We believe that these intermediates are involved in the amplification process, and as such offer the first example of the involvement of a rolling circle in the replication of eukaryotic DNA.
منابع مشابه
Rtt109 Prevents Hyper-Amplification of Ribosomal RNA Genes through Histone Modification in Budding Yeast
The genes encoding ribosomal RNA are the most abundant in the eukaryotic genome. They reside in tandem repetitive clusters, in some cases totaling hundreds of copies. Due to their repetitive structure, ribosomal RNA genes (rDNA) are easily lost by recombination events within the repeated cluster. We previously identified a unique gene amplification system driven by unequal sister-chromatid reco...
متن کاملRolling Circle Amplification (RCA): an approach for quick detection and identification of fungal species
Conventional methods for fungal identification in the clinical laboratory rely on morphological andphysiological tests. These tests often need several days or weeks to complete and are frequentlyunspecific. Molecular identification mostly implies sequencing, which is relatively expensive andtime-consuming, as well as impractical for large numbers of isolates. The Rolling CircleAmplification app...
متن کاملGenetic Analyses using Rolling Circle or PCR Amplified Padlock Probes
ABBREVIATIONS bp base pair cDNA complementary DNA CV coefficient of variation DNA deoxyribonucleic acid FRET fluorescence resonance energy transfer HRCA hyperbranched rolling circle amplification LN lymph node nt nucleotide OLA oligonucleotide ligation assay PCR polymerase chain reaction RCA rolling circle amplification RCR rolling circle replication RNA ribonucleic acid SCA serial circle ampli...
متن کاملCommon Commensal Cancer Viruses
The human microbiome—more properly called the bacteriome—has moved from the backwaters of microbiology to the forefront of clinical science and opened new approaches to diseases, including autoimmune disorders, cancer, and malnutrition. Human virome analyses, however, are at a nascent stage and disadvantaged by the necessity for detecting viruses (without highly conserved consensus sequences su...
متن کاملChemiluminescence detection of DNA/microRNA based on cation-exchange of CuS nanoparticles and rolling circle amplification.
Cation-exchange-based chemiluminescence amplification (CXCLAmp) was developed in this study. After amplification by rolling circle amplification (RCA), the proposed RCA-CXCLAmp strategy was used to detect miRNA sensitively.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 70 10 شماره
صفحات -
تاریخ انتشار 1973